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minimum essential medium alpha medium (α-mem) media  (Thermo Fisher)


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    Thermo Fisher minimum essential medium alpha medium (α-mem) media
    Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed <t>in</t> <t>α-MEM</t> medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.
    Minimum Essential Medium Alpha Medium (α Mem) Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/minimum+essential+medium+alpha+medium+(%CE%B1-mem)+media/pmc09279817-82-19-25
    Average 90 stars, based on 1 article reviews
    minimum essential medium alpha medium (α-mem) media - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "MiR-20a: a mechanosensitive microRNA that regulates fluid shear stress-mediated osteogenic differentiation via the BMP2 signaling pathway by targeting BAMBI and SMAD6"

    Article Title: MiR-20a: a mechanosensitive microRNA that regulates fluid shear stress-mediated osteogenic differentiation via the BMP2 signaling pathway by targeting BAMBI and SMAD6

    Journal: Annals of Translational Medicine

    doi: 10.21037/atm-22-2753

    Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed in α-MEM medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.
    Figure Legend Snippet: Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed in α-MEM medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.

    Techniques Used: Over Expression, Cell Differentiation, Transfection, Control, Quantitative RT-PCR, Activity Assay, Staining, Negative Control, Real-time Polymerase Chain Reaction

    Related Articles

    other:

    Article Title: Salidroside alleviates simulated microgravity-induced bone loss by activating the Nrf2/HO-1 pathway
    Article Snippet: The cells were cultured in alpha minimum essential media (α-MEM) supplemented with 10% fetal bovine serum (Gibco, USA) and 1% penicillin‒streptomycin (Gibco, China) at 37 °C with 5% CO 2 in a humidified incubator.

    Article Title: Hypoxic Extracellular Matrix Preserves Its Competence after Expansion of Human MSCs under Physiological Hypoxia In Vitro.
    Article Snippet: After thawing, MSCs were cultured in Petri dishes with minimum essential media alpha (α-MEM) (Gibco, Thermo Fisher Scientific, Horsham, UK) supplemented with penicillin/streptomycin (100 units/mL and 100 mg/mL, respectively (PanEco, Moscow, Russia)) and fetal bovine serum (10% vol/vol, (HyClone Laboratories Inc., South Logan, UT, USA)).

    Article Title: In Vitro and In Vivo Evaluation of 3D Printed Poly(Ethylene Glycol) Dimethacrylate-Based Photocurable Hydrogel Platform for Bone Tissue Engineering.
    Article Snippet: Alpha minimum essential media (α-MEM), fetal bovine serum (FBS), PBS, Dulbecco’s phosphate-buffered saline (DPBS), and penicillin/streptomycin were purchased from Gibco, Life Technologies (Thermo Fisher Scientific, USA).

    Article Title: Bioactive Matrix Scaffold of Oxidized Sacchachitin via Green Catalyst of SiliaCat TEMPO for Enhancing Bone Regeneration
    Article Snippet: Minimum essential media-alpha (MEM-α) (1, Gibco, Waltham, MA, USA), fetal bovine serum (FBS) (Gibco, InvivoGen, Waltham, MA, USA), penicillin-streptomycin solution as the antibiotic solution (Corning, Corning, NY, USA), and formaldehyde solution (10% (w/v) in aqueous phosphate buffer (Marcon, Radnor, PA, USA) were used as received without further purification.

    Article Title: Exploring the impact of oral bacteria remnants on stem cells from the Apical papilla: mineralization potential and inflammatory response
    Article Snippet: Cultivated bacteria were individually resuspended in the antibiotic-free cell culture media Minimum Essential Medium alpha (MEM α, GlutaMAXTM Supplement, no nucleosides, ThermoFisher, 32561-029) supplemented with 10% Fetal Bovine Serum (FBS; Sigma Aldrich, F7524).

    Article Title: MiR-20a: a mechanosensitive microRNA that regulates fluid shear stress-mediated osteogenic differentiation via the BMP2 signaling pathway by targeting BAMBI and SMAD6
    Article Snippet: Cell culture Culturing of MC3T3-E1 cells, a murine pre-osteoblastic cell line (ATCC, Manassas, VA, USA) was performed in minimum essential medium alpha medium (α-MEM) media (Life Technologies, Grand Island, NY, USA) with 1% penicillin/streptomycin (Life Technologies) and fetal bovine serum (FBS; 10%, Life Technologies).

    Cell Culture:

    Article Title: Hijacking the Cellular Mail: Exosome Mediated Differentiation of Mesenchymal Stem Cells
    Article Snippet: .. Cells were purchased from ATCC and cultured in growth media containing minimum essential medium alpha ( α -MEM) (Gibco), 20% fetal bovine serum (FBS) (Gibco), 1% L-glutamine (Gibco), and 1% antibiotic-antimycotic solution (anti-anti, Gibco). ..

    Article Title: Effects of 17β-Estradiol on Mitophagy in the Murine MC3T3-E1 Osteoblast Cell Line is Mediated via G Protein-Coupled Estrogen Receptor and the ERK1/2 Signaling Pathway
    Article Snippet: .. Cell culture media, minimum essential medium-alpha (α-MEM) and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). .. 17β-estradiol was obtained from Sigma-Aldrich (St. Louis, MO, USA).



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    minimum essential medium alpha medium (α-mem) media  (Thermo Fisher)
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    Thermo Fisher minimum essential medium alpha medium (α-mem) media
    Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed <t>in</t> <t>α-MEM</t> medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.
    Minimum Essential Medium Alpha Medium (α Mem) Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/minimum+essential+medium+alpha+medium+(%CE%B1-mem)+media/pmc09279817-82-19-25
    Average 90 stars, based on 1 article reviews
    minimum essential medium alpha medium (α-mem) media - by Bioz Stars, 2026-09
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    		  Buy from Supplier

    Image Search Results


    Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed in α-MEM medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.

    Journal: Annals of Translational Medicine

    Article Title: MiR-20a: a mechanosensitive microRNA that regulates fluid shear stress-mediated osteogenic differentiation via the BMP2 signaling pathway by targeting BAMBI and SMAD6

    doi: 10.21037/atm-22-2753

    Figure Lengend Snippet: Overexpression of miR-20a activates osteogenic MC3T3-E1 cell differentiation. After transfecting the cells with miR-20a double-stranded miRNA mimics (50 or 100 nM), miRNA NC (50 or 100 nM), or transfection reagent only (control) for 12 h, the cultures of confluent MC3T3-E1 cells were placed in α-MEM medium with β-glycerophosphate (10 mM) and 50 µg/mL ascorbic acid. (A) qRT-PCR assays for ALP mRNA at 24 h. (B) Detection of ALP activity on day 3. (C) Determination of ECM mineralization by stained with Alizarin red S on day 14. Relative means and standard deviations were shown underneath (P<0.001; scale bar: 50 µm). Data are expressed as mean ± SD for n=3; **, P<0.01 vs. NC; ***, P<0.001 vs. NC. NC, negative control; qRT-PCR, real-time quantitative polymerase chain reaction; ALP, alkaline phosphatase; ECM, extracellular matrix.

    Article Snippet: Cell culture Culturing of MC3T3-E1 cells, a murine pre-osteoblastic cell line (ATCC, Manassas, VA, USA) was performed in minimum essential medium alpha medium (α-MEM) media (Life Technologies, Grand Island, NY, USA) with 1% penicillin/streptomycin (Life Technologies) and fetal bovine serum (FBS; 10%, Life Technologies).

    Techniques: Over Expression, Cell Differentiation, Transfection, Control, Quantitative RT-PCR, Activity Assay, Staining, Negative Control, Real-time Polymerase Chain Reaction